Leukemia Q-Fusion BCR-ABL1 Genotyping and Quantification Kits

A novel multiplex system for simultaneous detection of BCR-ABL1 p190, p210, and p230 variants in leukemia patients.

Many hematologic malignancies carry characteristic chromosomal translocations. Correct identification of fusion genes can ensure an accurate diagnosis and prognosis is made and can assist in making treatment decisions, stratification, and disease monitoring. Current methodologies used to detect fusion genes can be laborious and time-consuming, typically involving karyotyping, followed by fluorescence in situ hybridization (FISH) and molecular analysis based on reverse transcriptase polymerase chain reaction (RT-PCR).

The gene sequence called BCR-ABL1 confirms the diagnosis of CML, and a form of acute lymphoblastic lymphoma (ALL) called Philadelphia chromosome (Ph)-positive ALL. Once CML or Ph-positive ALL has been diagnosed, BCR-ABL1 quantitative genetic testing is ordered periodically (typically every 3 months) to monitor the response to treatment and monitor for recurrence.

Key Features:

  • Multiplexing: Allows simultaneous detection of all BCR-ABL1 gene variants.
  • Comprehensiveness: The follow-up quantification kits are available to detect the corresponding variants for MRD monitoring.
  • Compatibility: Compatible with most current real-time qPCR instruments.
  • Rapid: Results can be obtained in 2-3 hours.
  • Simple: Easy to perform for routine screening.
  • Cost Effective: Can be performed for a fraction of the cost of FISH.

Disease Relevance:

  • Chronic Myeloid Leukemia (CML)
  • Ph-positive ALL


Technical Specs:

Fusion genes BCR-ABL1 p190, p210, and p230
Sample types Bone marrow, peripheral blood, FFPE, and cell lines
Reaction Volume 25 ul
Reaction time 2-3 hour
Internal control GUSB as housekeeping genes, 2 positive control, 3 negative control
Sensitivity 10-4
Instruments Compatible with Bio-Rad CFX96, iCycler, ABI7500, Stratagene Mx3000P/Mx3005P, and other commonly used RT-qPCR machines.


Ordering Information:

Cat. No. Description Size
1-100-13020 Q-Fusion BCR-ABL1 Genotyping Kit 20-reactions
1-100-14020 Q-Fusion BCR-ABL1 p190 Quantification Kit 20-reactions
1-100-15020 Q-Fusion BCR-ABL1 p210 Quantification Kit 20-reactions

 

The BCR-ABL1 Genotyping and Quantification Test kit is a CE-marked product for In Vitro Diagnostic Use.
The BCR-ABL1 Genotyping and Quantification Test kit is not FDA-cleared.

Contact Us  :    1-855-QuanDx-Care (1-855-782-6392)   OR   650-262-4140       info@quandx.com     Product Inquiry
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