Leukemia Q-Fusion PML-RARα Genotyping and Quantification Kits

A novel multiplex system for simultaneous detection of PML-RARα L, PML-RARα S, and PML-RARα V variants in leukemia patients.

Many hematologic malignancies carry characteristic chromosomal translocations. Correct identification of fusion genes can ensure an accurate diagnosis and prognosis is made and can assist in making treatment decisions, stratification, and disease monitoring. Current methodologies used to detect fusion genes can be laborious and time-consuming, typically involving karyotyping, followed by fluorescence in situ hybridization (FISH) and molecular analysis based on reverse transcriptase polymerase chain reaction (RT-PCR).

Up to 98% of cases of acute promyelocytic leukemia (APL), a subtype of AML, have a characteristic t(15;17) PML-RARα reciprocal chromosomal translocation.

Key Features:

  • Multiplexing: Allows simultaneous detection of all PML-RARα L, PML-RARα S, and PML-RARα V gene variants.
  • Comprehensiveness: The follow-up quantification kits are available to detect the corresponding variants for MRD monitoring.
  • Compatibility: Compatible with most current real-time qPCR instruments.
  • Rapid: Results can be obtained in 2-3 hours.
  • Simple: Easy to perform for routine screening.
  • Cost Effective: Can be performed for a fraction of the cost of FISH.

Disease Relevance:

  • Acute Promyelocytic Leukemia (APL), a subtype of AML

Technical Specs:

Fusion genes PML-RARα L, PML-RARα S, and PML-RARα V variants
Sample types Bone marrow, peripheral blood, FFPE, and cell lines
Reaction Volume 25 ul
Reaction time 2-3 hour
Internal control GUSB as housekeeping genes, 2 positive control, 3 negative control
Sensitivity 10-4
Instruments Compatible with Bio-Rad CFX96, iCycler, ABI7500, Stratagene Mx3000P/Mx3005P, and other commonly used RT-qPCR machines.

Ordering Information:

Cat. No. Description Size
1-100-16020 Q-Fusion PML-RARα Genotyping Kit 20-reactions
1-100-17020 Q-Fusion PML-RARα L Quantification Kit 20-reactions
1-100-18020 Q-Fusion PML-RARα S Quantification Kit 20-reactions
1-100-19020 Q-Fusion PML-RARα V Quantification Kit 20-reactions

 

The Leukemia Q-Fusion PML-RARα Genotyping and Quantification Kit is a CE-marked product for In Vitro Diagnostic Use.
The Leukemia Q-Fusion PML-RARα Genotyping and Quantification Kit is not FDA-cleared.

Contact Us  :    1-855-QuanDx-Care (1-855-782-6392)   OR   650-262-4140       info@quandx.com     Product Inquiry
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