Many hematologic malignancies carry characteristic chromosomal translocations. Correct identification of fusion genes can ensure an accurate diagnosis and prognosis is made and can assist in making treatment decisions, stratification, and disease monitoring. Current methodologies used to detect fusion genes can be laborious and time-consuming, typically involving karyotyping, followed by fluorescence in situ hybridization (FISH) and molecular analysis based on reverse transcriptase polymerase chain reaction (RT-PCR).
Up to 98% of cases of acute promyelocytic leukemia (APL), a subtype of AML, have a characteristic t(15;17) PML-RARα reciprocal chromosomal translocation.
Fusion genes | PML-RARα L, PML-RARα S, and PML-RARα V variants |
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Sample types | Bone marrow, peripheral blood, FFPE, and cell lines |
Reaction Volume | 25 ul |
Reaction time | 2-3 hour |
Internal control | GUSB as housekeeping genes, 2 positive control, 3 negative control |
Sensitivity | 10-4 |
Instruments | Compatible with Bio-Rad CFX96, iCycler, ABI7500, Stratagene Mx3000P/Mx3005P, and other commonly used RT-qPCR machines. |
Cat. No. | Description | Size |
1-100-16020 | Q-Fusion PML-RARα Genotyping Kit | 20-reactions |
1-100-17020 | Q-Fusion PML-RARα L Quantification Kit | 20-reactions |
1-100-18020 | Q-Fusion PML-RARα S Quantification Kit | 20-reactions |
1-100-19020 | Q-Fusion PML-RARα V Quantification Kit | 20-reactions |
The Leukemia Q-Fusion PML-RARα Genotyping and Quantification Kit is a CE-marked product for In Vitro Diagnostic Use.
The Leukemia Q-Fusion PML-RARα Genotyping and Quantification Kit is not FDA-cleared.
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